Part:BBa_K2308004:Design

used to knock out the gene crtB in the genome of Rhodobacter Sphaeroides+ Amp

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 93
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 47
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1250
  Illegal BsaI.rc site found at 162
  Illegal SapI site found at 1759
  

Design Notes

There's a point mutant in the upstream sequence (in order to avoid endonuclease site) so the efficiency of homologous recombination will be affected/

Source

The up/downstream sequences are from Rhodobacter Sphaeroides.